IMT 16-2024 Master Thesis High-Throughput Microchip NMR

Organisationseinheit

Institut für Mikrostrukturtechnik (IMT)

Ihre Aufgaben

Pharmaceutical research increasingly depends on microfluidics-based advanced in-vitro models (AIM) based on sophisticated cultures of cells and cell aggregates for pre-clinical testing. Since they can use human cells, AIM are potentially more accurate than animal models. Their introduction is also driven by the recognised need to reduce animal testing. Gaining useful information from AIM is a challenge, as analytical techniques must be at the same time highly informative and keep the cells alive and functional. Nuclear Magnetic Resonance (NMR) spectroscopy provides detailed, non-invasive, real-time insights into AIM life processes but is currently limited to single sample observation. This project aims to efficiently analyse multiple samples to enable high-throughput operation.

The objective of this Master project is to develop and implement a multisample analysis capability for AIM by NMR, allowing for simultaneous and detailed monitoring of life processes in multiple in vitro models.

The project will develop in three phases:

• Develop and produce a microfluidic insert that integrates with commonly used biological culturing platforms.
• Design and build a prototype of the insert injector system for integration into an NMR spectrometer.
• Conduct a proof-of-concept study to showcase the high-throughput capabilities of the system within an NMR spectrometer environment.

It is expected that the successful project leads to at least one publication in an internationally peer-reviewed journal.

Eintrittstermin

as soon as possible

Ihre Qualifikation

You are an ambitious mechanical engineer passionate about product design. You should possess proficiency in 3D modeling and practical expertise in programming microcontrollers.